Table 1 Genes with significantly increased hypothalamic expression between mice fed high fat diets relative to low fat fed controls, identified by edgeR (FDR < 0.05).

From: Diet induced obesity is independent of metabolic endotoxemia and TLR4 signalling, but markedly increases hypothalamic expression of the acute phase protein, SerpinA3N

Gene Name

Fold change

Log fold change

P-value

FDR

Gene description*

Serpina3n

1.7

−0.793236193

5.06E-09

8.52E-05

Serine (or cysteine) peptidase inhibitor

LOC10524359

62.8

−5.972717972

1.87E-07

0.001571933

60S ribosomal protein L23a pseudogene

Stat3

1.2

−0.266379652

1.01E-06

0.005687007

Signal transducer and activator of transcription 3

Gm6210

14.5

−3.86002715

3.88E-06

0.016325199

Heat shock protein 8 pseudogene

Socs3

1.6

−0.655744451

9.62E-06

0.032403367

Suppressor of cytokine signalling 3

GM15459

8.7

−3.112752289

1.44E-05

0.040307169

Heat shock protein 8 pseudogene

  1. *As given by NCBI Gene Database.
  2. Differential expression of 2 of the known genes, Serpina3n and Socs3, was verified using in situ hybridisation (Fig. 4). Serpina3n gene is expressed in the arcuate (ARC) and dorsomedial (DMH) nucleus regions of the hypothalamus. After 20 weeks of HFD, Serpina3n gene expression was significantly increased in both regions compared to mice on LFD, although the magnitude of the change is greatest in the DMH (Fig. 4A,C). Similarly, Socs3 gene expression within the hypothalamus also increased by 20 weeks of HFD (Fig. 4B,D).
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