Figure 5

Analysis of markers of HSC activation in livers from Fc-GLP-1 treated mice 2 weeks after PH. (a) Hepatic mRNA levels of HSC related genes analyzed 2 weeks after PH (n = 6 mice/group). (b,c) Representative images and quantification of α-SMA and desmin immunohistochemistry (n = 4 mice/group; 3 random fields per mice; *p = 0.018 (C+Fc-GLP-1); **p = 0.00014 (MCD) and **p = 0.00019 (M+Fc-GLP-1) and Sirius Red staining (2 fields per mice; n = 4 mice/group) at 2 weeks after PH. (d) Representative TEM images of livers from M+Fc-GLP-1 mice showing hepatic stellate cells (HSC) containing lipid droplets (LD) and collagen fibers (COL) surrounding vein (V) and quantification of HSC (4 random fields per mice; n = 3 mice/group). *p < 0.05, **p < 0.01, ***p < 0.001 vs C group; ##p < 0.01, ###p < 0.001 vs MCD group according to one-way ANOVA with post-hoc Bonferroni test.