Figure 6

Comparison of the microarray data of livers from Fc-GLP-1 treated mice before and after PH by GSEA software. (a) Venn diagram of gene sets up-regulated in C+Fc-GLP-1 and M+Fc-GLP-1 groups 2 weeks after PH versus values of each individual group analyzed in liver samples removed in the hepatectomy (n = 4 mice/group). (b) Gene sets commonly up-regulated in both C+Fc-GLP-1 and M+Fc-GLP-1 groups versus values analyzed in liver samples removed in the hepatectomy. (c) Principal inflammatory gene sets up-regulated in Fc-GLP-1 treated animals 2 weeks post-PH versus values obtained in liver samples removed in the hepatectomy (n = 4 mice/group). (d) mRNA levels of pro-inflammatory cytokines, cytotoxic lymphocytes and neutrophils related genes analyzed 2 weeks after PH in comparison with values of each individual group measured in liver samples removed in the hepatectomy (n = 6 mice/group). (e) Principal HSC-related gene sets up-regulated in Fc-GLP-1 treated animals as indicated above (n = 4 mice/group). (f) mRNA levels of genes related to HSC activation in Fc-GLP-1- treated animals as indicated above (n = 6 mice/group). Fold change of hepatic levels of metabolites related to activation of HSC in C+ Fc-GLP-1 (g) and M+ Fc-GLP-1 (h) under conditions indicated above (n = 6 mice/group). *p < 0.05, **p < 0.01, ***p < 0.001 vs each group before PH according to one-way ANOVA with post-hoc Bonferroni test.