Figure 8 | Scientific Reports

Figure 8

From: Melatonin suppresses TLR9-triggered proinflammatory cytokine production in macrophages by inhibiting ERK1/2 and AKT activation

Figure 8

Melatonin inhibits TLR9-mediated proinflammatory cytokines production in vivo. C57BL6/J mice were intraperitoneally injected with thioglycollate (TG) to elicite macrophages on day 0 and then received 5 mg/kg melatonin (MT) or ethanol (E) on day 3 and day 4. On day 4, mice were further stimulated by intraperitoneal injection of 50 μg CpG-ODN two hours after melatonin pretreatment. TNF-α (A) and IL-6 (B) in serum of mice in various groups (n = 5) were assayed by ELISA 2 hours after CpG-ODN injection. Error bars represent s.d. **P < 0.01 (ANOVA).

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