Figure 7
From: ERK1/2-dependent gene expression in the bovine ovulating follicle
Intrafollicular injection of PD0325901 reduces the relative mRNA abundance of LH-induced ovulatory genes in granulosa cells. Relative mRNA abundance of EGR1, ADAMTS1, TNFAIP6, STAT3, and TIMP1 in granulosa cells of cows in Vehicle, Vehicle + GnRH and PD0325901 + GnRH groups (N = 3–6/time-point). Cows were subject to follicular-wave synchronization and were challenged with Vehicle or PD0325901 30 minutes prior intramuscular administration to GnRH. Granulosa cell were collected from groups of individual cows at 0 h and 6 h relative to GnRH stimulus. Transcript abundance of each gene was normalized to reference genes ACTB, L19, and CYCLOPHILIN. All data are expressed as a mean ± S.E.M, where different letters (A&B) represent differences at p < 0.05 and C represents tendency for differences (P < 0.1) after a one-way ANOVA.
