Figure 8
From: ERK1/2-dependent gene expression in the bovine ovulating follicle
The impact of ERK1/2 pathway inhibition by PD0325901 on gene transcription in bovine theca cells. In the presence of PD0325901 following GnRH stimulation TIMP1 is reduced in theca cells (A), while ADAMTS1 and STAT3 are induced (B). In bovine theca cells, EGR1 and TNFAIP6 remain unchanged between Vehicle + GnRH and PD0325901 + GnRH. Data were normalized to reference genes ACTB, L19, and CYCLOPHILIN (C). All data are expressed as a mean ± S.E.M, where * represents p < 0.05.
