Figure 9
From: ERK1/2-dependent gene expression in the bovine ovulating follicle
The impact of ERK1/2 pathway inhibition by PD0325901 on the transcription and translation of the steroidogenic enzyme STAR. (A) Relative mRNA abundance of STAR comparing Vehicle + GnRH and PD0325901 + GnRH. (B) STAR protein abundance by immunoblot in bovine granulosa cells subject to PD032590. EGR1 was used as a time-point control and ACTINB was used as a loading control. The blot was cut and cropped to analyze all three proteins by antibodies on the same blot. (C) Densitometry reveals quantitative abundance of STAR protein abundance in GnRH stimulated bovine granulosa cells in the presence of PD0325901. (D) RT-qPCR was performed on theca cells collected from cattle subject to GnRH treatment in the presence or absence of PD0325901 to establish the expression pattern of STAR. Transcript data were normalized to reference genes ACTB, L19, and CYCLOPHILIN. All data are expressed as a mean ± S.E.M, where letters (A&B) and * represents p < 0.05 and ** represents p < 0.01.
