Table 3 Non-synonymous SNPs detected in the 73 genes related to antibiotic resistance in the 22 isolates studied using PAO1 as the reference genome.
Gene locus | Gene name | Mechanism | P. aeruginosa strains/number of SNPs | ||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
PA31 | PA32 | PA33 | PA34 | PA35 | PA37 | PA82 | PA17 | PA40 | PA149 | PA157 | PA171 | PA175 | PA55 | PA57 | PA59 | PA64 | PA66 | PA86 | PA92 | PA100 | PA102 | Total SNPs | |||
PA0156 | triA | Antibiotic efflux | 4 | 5 | 5 | 1 | 6 | 5 | 4 | 1 | Â | 1 | Â | Â | 3 | Â | 1 | Â | Â | Â | Â | Â | Â | 1 | 37 |
PA0157 | triB | Â | Â | 1 | Â | Â | Â | 1 | 1 | Â | 2 | 1 | Â | Â | Â | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 14 | |
PA0158 | triC | 2 | 2 | 2 | 1 | 2 | 2 | 1 | Â | Â | 1 | Â | Â | 1 | Â | 3 | Â | Â | Â | Â | Â | Â | 1 | 18 | |
PA0424 | mexR | 2 | 1 | 1 | 2 | 1 | 1 | 1 | Â | Â | Â | Â | Â | 1 | Â | 2 | Â | Â | Â | Â | Â | Â | Â | 12 | |
PA0425 | mexA | Â | Â | Â | Â | Â | Â | Â | Â | 1 | Â | 1 | Â | 1 | Â | Â | 1 | Â | 1 | 1 | Â | 1 | Â | 7 | |
PA0426 | mexB | 1 | 1 | 2 | Â | 1 | 4 | Â | Â | Â | Â | Â | Â | Â | Â | 2 | Â | 1 | Â | Â | 1 | Â | Â | 13 | |
PA0427 | oprM | Â | Â | Â | Â | Â | Â | Â | Â | Â | 1 | 1 | Â | Â | Â | Â | 1 | Â | 1 | 1 | Â | Â | Â | 5 | |
PA1236 | farB | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | Â | Â | 1 | Â | Â | Â | Â | Â | 1 | Â | Â | 1 | Â | Â | 11 | |
PA1282 | lrfA | 6 | 6 | 9 | 4 | 6 | 8 | 6 | 8 | 5 | 5 | 6 | 4 | 4 | Â | 7 | 3 | 4 | 3 | 3 | 4 | 3 | 4 | 108 | |
PA1316 | lrfA | 2 | 2 | 2 | 1 | 2 | 2 | 3 | 3 | 2 | 1 | 3 | 2 | 3 | Â | 3 | 2 | 2 | 2 | 2 | 2 | 2 | 4 | 47 | |
PA1435 | mexM | 4 | 4 | 4 | 5 | 4 | 4 | 3 | 4 | 6 | 5 | 6 | 6 | 5 | Â | 8 | 5 | 5 | 5 | 5 | 5 | 4 | 5 | 102 | |
PA1436 | mdtC | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 3 | 2 | Â | 3 | 2 | 2 | Â | Â | 2 | 2 | 2 | 2 | 2 | 2 | 4 | 41 | |
PA1754 | cysB | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 1 | Â | 1 | |
PA2018 | mexY | 5 | 5 | 5 | 5 | 5 | 5 | 3 | 1 | 1 | 1 | 4 | 1 | 3 | Â | 3 | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 55 | |
PA2019 | mexX | 4 | 4 | 4 | 4 | 4 | 4 | 5 | 3 | 4 | 5 | 3 | 3 | 3 | Â | 6 | 3 | 4 | 3 | 3 | 4 | 3 | 4 | 80 | |
PA2389 | macA | 2 | 1 | 1 | 1 | 1 | 1 | 1 | Â | 1 | Â | 1 | 1 | 1 | Â | 1 | 1 | Â | 1 | 1 | Â | 1 | 1 | 18 | |
PA2390 | macB | 1 | 1 | 1 | 3 | 1 | 1 | 2 | 1 | 3 | 2 | 3 | 1 | 1 | Â | 3 | 1 | 2 | 1 | 1 | 2 | 1 | 3 | 35 | |
PA2391 | opmQ | 6 | 5 | 6 | 4 | 6 | 6 | 4 | 1 | 3 | 2 | 1 | 2 | 4 | Â | 5 | 4 | 5 | 4 | 4 | 5 | 3 | 1 | 81 | |
PA2491 | mexS | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 1 | 1 | Â | 4 | 2 | 2 | 2 | 2 | 1 | 1 | 1 | 34 | |
PA2493 | mexE | Â | Â | Â | 1 | Â | Â | Â | 1 | Â | Â | Â | 1 | 3 | Â | 2 | 1 | 2 | 1 | 1 | 1 | Â | Â | 14 | |
PA2494 | mexF | Â | Â | Â | 1 | Â | Â | 1 | Â | Â | Â | Â | Â | Â | Â | 2 | Â | Â | Â | Â | Â | Â | Â | 4 | |
PA2495 | oprN | 1 | 1 | 1 | 2 | 1 | 1 | 1 | Â | 1 | 1 | Â | Â | 1 | Â | 2 | Â | 1 | Â | Â | 1 | Â | Â | 15 | |
PA2525 | adeC | Â | Â | Â | 3 | Â | Â | Â | Â | Â | 5 | 1 | Â | 1 | Â | 2 | 2 | Â | Â | 2 | Â | 3 | 1 | 20 | |
PA2526 | muxC | Â | Â | Â | Â | Â | Â | Â | Â | 1 | Â | Â | 1 | Â | Â | 1 | 1 | Â | 1 | 1 | Â | Â | Â | 6 | |
PA2527 | muxB | Â | Â | Â | 1 | Â | Â | Â | Â | 2 | Â | Â | Â | 1 | Â | Â | Â | Â | Â | Â | Â | Â | Â | 4 | |
PA2837 | opmA | 3 | 3 | 3 | 4 | 3 | 3 | 5 | Â | 1 | 1 | Â | 3 | 4 | Â | 6 | 2 | 1 | 2 | 2 | 1 | 1 | 2 | 50 | |
PA3019 | taeA | 1 | 1 | 1 | 3 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 2 | 2 | Â | 4 | 1 | 1 | 1 | 1 | 1 | 3 | 1 | 32 | |
PA3137 | farB | 1 | 1 | 1 | 1 | 1 | 1 | Â | 1 | 2 | Â | 2 | 2 | 2 | Â | 2 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 24 | |
PA3521 | opmE | 3 | 3 | 3 | 2 | 3 | 3 | 2 | 3 | 5 | 6 | 4 | 2 | 3 | Â | 11 | 3 | 5 | 3 | 3 | 5 | 3 | 2 | 77 | |
PA3522 | mexQ | 4 | 4 | 4 | 4 | 4 | 4 | 6 | 4 | 5 | 2 | 2 | 3 | 3 | Â | 3 | 4 | 6 | 4 | 4 | 5 | 2 | 3 | 80 | |
PA3523 | mexP | 2 | 2 | 2 | 1 | 2 | 2 | 2 | 3 | 2 | Â | 2 | 1 | 2 | Â | 3 | Â | 3 | Â | Â | 3 | 2 | 2 | 36 | |
PA3574 | nalD | Â | Â | Â | Â | Â | Â | Â | 1 | Â | Â | Â | 1 | Â | Â | Â | Â | 3 | Â | Â | Â | Â | Â | 5 | |
PA3676 | mexK | 1 | 1 | 1 | 2 | 1 | 1 | 5 | 1 | 2 | Â | 2 | 2 | 3 | Â | 6 | 4 | 2 | 4 | 4 | 2 | 2 | 2 | 48 | |
PA3677 | mexJ | 2 | 2 | 2 | 2 | 2 | 2 | 2 | Â | Â | 1 | Â | Â | 2 | Â | 3 | 3 | 3 | 3 | 3 | 3 | 1 | Â | 36 | |
PA3678 | mexL | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | Â | 1 | 1 | Â | 1 | Â | 2 | 2 | 1 | 2 | 2 | 1 | Â | Â | 21 | |
PA3894 | adeC | Â | Â | Â | 1 | Â | Â | 1 | Â | Â | 1 | 1 | 1 | Â | Â | 2 | 2 | 1 | 2 | 2 | 1 | 1 | Â | 16 | |
PA4205 | mexG | 1 | 1 | 1 | Â | 1 | 1 | 1 | Â | Â | Â | Â | Â | Â | Â | 3 | Â | Â | Â | Â | Â | Â | Â | 9 | |
PA4206 | mexH | 1 | 1 | 1 | 2 | 1 | 1 | Â | Â | Â | Â | Â | Â | 1 | Â | 1 | Â | Â | Â | Â | Â | Â | Â | 9 | |
PA4207 | mexI | 1 | 1 | 1 | 1 | 1 | 1 | 1 | 1 | Â | Â | 1 | 1 | 1 | Â | Â | 1 | 1 | 1 | 1 | 1 | Â | Â | 16 | |
PA4208 | opmD | 3 | 3 | 3 | 3 | 3 | 3 | 2 | 5 | 1 | 1 | Â | Â | 4 | Â | 6 | Â | Â | Â | Â | Â | Â | 1 | 38 | |
PA4374 | mexV | 2 | 2 | 2 | 4 | 2 | 2 | 3 | Â | 2 | 1 | 1 | 2 | 2 | Â | 4 | 1 | 2 | 1 | 1 | 2 | 1 | 3 | 40 | |
PA4375 | mexW | 2 | 2 | 2 | 1 | 2 | 2 | 3 | 3 | 1 | 2 | 3 | 1 | 1 | Â | 2 | 1 | 1 | 1 | 1 | 1 | Â | Â | 32 | |
PA4595 | yjjk | Â | Â | Â | 1 | Â | Â | Â | 1 | Â | 2 | Â | 1 | 2 | Â | 1 | 3 | 1 | 2 | 1 | 1 | Â | 1 | 17 | |
PA4597 | oprJ | Â | Â | Â | Â | Â | Â | Â | 2 | 2 | 2 | 2 | Â | Â | Â | 9 | 3 | 3 | 5 | 3 | 3 | 3 | Â | 37 | |
PA4598 | mexD | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 3 | 2 | 2 | 1 | 3 | 2 | Â | 9 | 2 | 1 | 2 | 2 | 1 | 2 | 3 | 49 | |
PA4599 | mexC | 7 | 8 | 8 | 3 | 8 | 8 | 6 | 4 | 1 | 4 | Â | 1 | 5 | Â | 9 | 1 | Â | 1 | 1 | Â | 4 | 4 | 83 | |
PA4600 | nfxB | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 1 | Â | Â | Â | 1 | Â | Â | Â | Â | Â | Â | Â | 2 | |
PA4974 | opmH | 2 | 1 | 1 | 1 | 5 | 5 | Â | Â | 1 | Â | Â | 2 | 1 | Â | 5 | 1 | 5 | 1 | 1 | 3 | 4 | Â | 39 | |
PA4990 | emrE | 1 | 1 | 1 | Â | 1 | 2 | Â | Â | Â | 1 | Â | Â | Â | Â | Â | Â | 1 | Â | Â | 1 | Â | Â | 9 | |
PA4997 | msbA | 2 | 3 | 3 | 2 | 2 | 3 | 4 | Â | Â | Â | Â | Â | 4 | Â | 3 | Â | 1 | Â | Â | 1 | Â | 1 | 29 | |
PA5158 | adeC | 3 | 3 | 3 | Â | 3 | 3 | 3 | 2 | 2 | 1 | 2 | Â | 3 | Â | 5 | 3 | 3 | 3 | Â | 3 | 2 | 1 | 48 | |
PA5160 | farB | 4 | 3 | 3 | 2 | 4 | 3 | 5 | 3 | 3 | 3 | 3 | 3 | 3 | Â | 3 | 3 | 4 | 3 | Â | 3 | 4 | 3 | 65 | |
PA5518 | rosB | 3 | 3 | 3 | 2 | 3 | 3 | 3 | Â | Â | Â | Â | 1 | 1 | Â | 1 | 1 | 1 | 1 | Â | 1 | 1 | 1 | 29 | |
PA0706 | catB7 | Antibiotic inactivation | 4 | 4 | 4 | 5 | 4 | 4 | 3 | 3 | 2 | 2 | 2 | 3 | 4 | Â | 1 | 2 | 4 | 2 | 2 | 4 | 3 | 3 | 65 |
PA1129 | fosA | Â | Â | Â | Â | Â | Â | Â | Â | 1 | Â | Â | Â | Â | Â | 2 | Â | Â | Â | Â | Â | Â | Â | 3 | |
PA4109 | ampR | 2 | 2 | 2 | 3 | 2 | 2 | 1 | Â | Â | Â | Â | 2 | 2 | Â | 3 | Â | Â | Â | Â | Â | Â | Â | 21 | |
PA4110 | ampC | 5 | 5 | 5 | 3 | 5 | 5 | 6 | 1 | 2 | 2 | 2 | 2 | 5 | Â | 12 | 1 | 1 | 1 | 1 | 1 | 2 | 2 | 69 | |
PA4119 | Aph(3′)-IIb | 2 | 2 | 2 | 3 | 2 | 2 | 2 | 1 | 1 | 1 | 1 | 4 |  |  | 3 |  | 1 |  |  | 1 | 1 | 2 | 31 | |
PA5514 | OXA-50 | 1 | 2 | Â | 1 | 3 | 2 | 5 | 2 | 3 | Â | 2 | 3 | 3 | Â | 4 | 1 | 3 | 1 | Â | 3 | Â | 4 | 43 | |
PA0004 | gyrB | Antibiotic target alternation | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 1 | 3 | 1 | Â | Â | 5 |
PA0903 | alaS | 1 | 1 | 1 | 1 | 1 | 1 | 1 | Â | 1 | Â | Â | Â | Â | Â | Â | 1 | Â | 1 | 1 | Â | 2 | Â | 13 | |
PA1972 | pmrC | 3 | 3 | 3 | 3 | 3 | 3 | 1 | 1 | 1 | 4 | 1 | 1 | 2 | Â | 3 | 2 | 3 | 2 | 2 | 3 | 4 | 1 | 49 | |
PA3002 | mfd | 1 | 2 | 2 | 3 | 2 | 2 | 1 | 1 | 1 | Â | 2 | Â | 1 | Â | 2 | 1 | 2 | 1 | 1 | 2 | Â | Â | 27 | |
PA3168 | gyrA | 1 | 1 | 1 | Â | 1 | 1 | Â | Â | Â | Â | Â | Â | 2 | Â | 2 | Â | 1 | Â | Â | Â | Â | 1 | 11 | |
PA3946 | rosC | 6 | 8 | 8 | 3 | 7 | 6 | 1 | 1 | 1 | 1 | 2 | 2 | Â | Â | Â | 1 | 2 | 1 | 1 | 2 | 2 | 1 | 56 | |
PA4265 | tufA | 1 | Â | Â | Â | Â | 1 | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 2 | |
PA4277 | tufB | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 0 | |
PA4560 | ileS | 2 | 2 | 2 | 2 | 2 | 2 | 2 | 1 | 4 | 2 | 1 | 1 | 2 | Â | 4 | 2 | 4 | 2 | 2 | 4 | 3 | 1 | 47 | |
PA4964 | parC | 2 | 2 | 2 | Â | 2 | 2 | Â | Â | 1 | Â | Â | Â | 1 | Â | 1 | Â | Â | Â | Â | Â | Â | Â | 13 | |
PA4967 | parE | 1 | 1 | 1 | 1 | 1 | 1 | 2 | 1 | Â | Â | Â | Â | 1 | Â | 1 | 1 | 1 | 1 | 1 | 2 | Â | 1 | 18 | |
PA3553 | pmrF | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | Â | 3 | 1 | Â | 1 | 1 | Â | 1 | Â | 7 | |
PA3554 | arnA | 2 | 4 | 4 | 5 | 4 | 4 | 3 | 2 | 3 | Â | 3 | Â | 4 | Â | 6 | Â | 2 | Â | Â | 2 | 3 | 4 | 55 | |
PA0920 | mprF | 6 | 6 | 6 | 4 | 6 | 6 | 9 | Â | 2 | 1 | 1 | 2 | 7 | Â | 8 | 2 | 1 | 2 | 2 | 1 | 2 | 2 | 76 | |
| Â | Â | Total SNPs | 137 | 140 | 144 | 124 | 146 | 150 | 136 | 82 | 89 | 79 | 83 | 77 | 125 | 0 | 217 | 88 | 109 | 88 | 81 | 101 | 87 | 86 | Â |