Figure 4

Microscopic studies: (A) Morphological analysis of MDA-MB231 under inverted microscope. (a,b) the cells exposed to IC50 of OEO and thymol for 24 h; with morphological alterations including loss of adhesion, rounding, and sporadic distribution (c) control cells; with typical shape and a growth pattern of patchy monolayer. (B) Fluorescence staining of MDA-MB231 cells (d,e) the cells exposed to IC50 of OEO and thymol for 24 h (f) control cells. The cells were stained using AO and EtBr and visualized with fluorescence microscopy.