Figure 4
From: Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours

Validation of the coupling of MagPlex microspheres with the recombinant halotag fused TAAs. The protein coupled microspheres were reacted individually with increasing dilution of corresponding protein specific antibodies. The MFI signals generated by individual protein coupled microspheres increased with increasing concentration of antibodies in concentration dependent fashion.