Figure 6
From: Multiplexed Autoantibody Signature for Serological Detection of Canine Mammary Tumours
Comparison of MFI values corresponding to autoantibody response (against five TAAs) in 50 healthy and 75 canine mammary tumour sera. (A–E) Scatter diagrams representing CMYC, MNSOD, PGAM1, TPI & MUC1 autoantibody response in canine mammary tumour (CMT) sera (n = 75) and healthy dog sera (n = 50). (F) At 1:200 dilution of sera samples, the multiplex assay comprising of 5 autoantigens, namely TPI, PGAM1, MUC1, MNSOD, & CMYC coupled microspheres, produced significantly higher average MFI signals in CMT sera (n = 75) compared to healthy dog sera samples (n = 50) (Mann-Whitney, p value < 0.0001). At cut-off value (avg. MFI of healthy sera + 2 SD), individual assays were found to be highly specific (96–98% specificity). The bars represent average MFI values and error bars represent standard error.
