Figure 2

Ligand-induced stabilization of G4-DNA (ΔT_½, °C) from FRET-melting experiments: (a) F-21G-T (K-10 buffer), (b) F-21G-T (Na-10 buffer), (c) F-21CTA-T (K-10 buffer), (d) F-Pu24T-T, (e) F-CEB25^WT-T, and (f) F-CEB25^L111-T (K-1 buffer), in the absence (black) or in the presence of 3 (gray) or 10 μM (light gray bars) of the duplex competitor (ds26). Conditions: c(F-G4-T) = 0.2 µM; c(ligand) = 1 µM; error bars represent the standard deviation from two experiments.