Figure 8

(a,b) Yield of products (red: DNA cleavage, black: covalent adduct) formed in photoreactions of ^BrU-22AG (5 µM) with (a) M-1PY and (b) M-2PY (25 µM each) in the presence of ct DNA competitor, in K⁺ conditions. Error bars represent standards deviation from two independent experiments. (c–e) PAGE analysis of photoreaction of M-1PY or M-2PY with (a) self-complementary duplex ^BrU-ds26; (b) stem-loop ^BrU-hp29; (c) mismatch-containing ^BrU-hp29^MM (the mismatched ^BrU residues are typed in red). Lanes 1 and 5: only DNA; lanes 2 and 6: DNA with UV; lanes 3 and 4: DNA and M-1PY with UV; lanes 7 and 8: DNA and M-2PY with UV (365 nm, 3 and 5 min, respectively); lane 9 in (e) DMS/piperidine sequencing. Conditions: c(DNA) = 5 µM, c(ligand) = 25 µM in K-100 buffer.