Figure 4
Expression analysis of different lengths of exogenous re-MaSp1. (a) The relative expression analysis of different lengths ofre-MaSp1in the PSGs of the 3rd day of the fifth instar larvae in G4 heterozygous transgenic lineages was performed by qRT-PCR. Mean ± SD values were derived from three independent replicate experiments. Gradient sodium dodecyl sulfate-polyacrylamide gel (5%-12%SDS-PAGE) analysis of the degummed silkworm cocoons was performed, followed by immunoblotting on nitrocellulose membranes; M: 250 kDa protein Marker; CK: the degummed silkworm cocoons of wild-type Lan10; (b,c) the degummed silkworm cocoons of MASP1-2-1 and MASP1-2-2 transgenic lineages; (b’,c’) the degummed silkworm cocoons of MASP1-12-1, MASP1-12-5, MASP1-12-8 and MASP1-12-10 transgenic lineages; (b”,c”) the degummed silkworm cocoons of MASP1-16-2, MASP1-16-6, MASP1-16-8 and MASP1-16-14 transgenic lineages. (d) Expression analysis of the expression of different lengths of re-MaSp1 relative to the Fib-L protein in homozygous G5 through grey analysis using the Gel-Pro-analyzer4 software. Mean ± SD was derived from three independent replicate experiments.
