Figure 2
From: The 3D architecture of a bacterial swarm has implications for antibiotic tolerance
Inhabitation, speeds and turning rates of WT and Che- bacteria in a 3D swarm. The swarms contain a mixture of fluorescently-labeled and unlabeled cells. The fluorescent cells were tracked by DI as described in Fig. 1C–H. (A,D) The occupancy of WT (JP1020) and Che- mutants (JP2529 and JP2531) across 15 levels in a region whose maximal colony height is ~40 μm (see Fig. 1B). PDF stands for probability density function, where the Y-axis is normalized to a sum of all the data points (>200,000). (B,E) The average speed in the x-y plane at different heights for cells tracked in A and D, respectively. (C,F) The average speed along the z-direction at different heights for cells tracked in A and D, respectively. Speeds were calculated using a Matlab program as described under Methods. Error bars indicate standard deviations from the mean. (G,H) PDF of curvatures and torsions for WT (>250,000 data points) and Che- mutants (>200,000 data points). See Movie S5. (I) The graph plotting the steps between significant curvatures and torsions events for WT yields a power law distribution. See the section on ‘Curvature and torsion of trajectories’ under Methods for a fuller discussion of the results in G-I.
