Figure 3
Cardiac hypertrophy developed in UUO mice 3 weeks after injury. (A) Representative images of hearts harvested from control mice and mice that underwent UUO. By measurement the heart is slightly enlarged in mice with UUO. (B) Cardiac mass as measured by the ratio of heart weight to body weight was significantly increased in the UUO mice. *P < 0.05 vs. Control. N = 12. (C) Immunofluorescence staining of cardiac tissues with WGA-conjugated FITC in the control and UUO mice. Significantly increased cardiomyocyte circumference was observed in the UUO mice by WGA staining. Graph (right) represents the relative size of cardiomyocytes in the UUO mice compared to controls. ***P < 0.001 vs. Control. Bar = 100 μm. Bar values represent means ± SEM (error bars). Statistical analyses were performed with t-tests. N = 8. (D) Representative immunofluorescence images of anti-CD-31 staining in the heart in control and UUO mice. Red fluorescence signal represents CD-31 and blue fluorescence signal represents DAPI. Graph represents quantification of cardiac vascular rarefaction. There was observed but not statistically significant cardiac vascular rarefaction in the UUO mice relative to the controls. P = 0.5980 vs. Control. Bar = 100 µm. Bar values represent means ± SEM (error bars). Statistical analyses were performed with t-tests. N = 8.
