Figure 8
Enalapril attenuates cardiac fibrosis in UUO/CKD mice. (A) Immunofluorescence staining revealed that Enalapril treatment reduced intercardiomyocytic accumulation of collagen type I (Col I) and interstitial deposition of fibronectin (FN) in UUO/CKD mice. Bar = 100 μm. (B) Quantified relative fluorescence intensity showed decreased expression of Col I and FN in Enalapril-treated UUO mice. The relative fluorescence intensity was measured using ImageJ. *P < 0.05 vs. Control, ***P < 0.001 vs. Control, #P < 0.05 vs. UUO, ##P < 0.01 vs. UUO. (C) Immunoblotting confirmed that Enalapril treatment inhibited the expression of FN and Col I in the hearts of UUO/CKD mice. The expression levels of fibronectin and collagen type I were normalized to the expression level of β-actin. Protein quantification was performed using ImageJ software. *P < 0.05 vs. Control, #P < 0.05 vs. UUO. (D) Reduced expression of FN and Col I in Enalapril-treated UUO/CKD mice was determined by qRT-PCR. The expression levels of fibronectin and collagen type I were normalized to the expression level of β-actin. *P < 0.05 vs. Control, ***P < 0.001 vs. Control, ###P < 0.001 vs. UUO. Values were expressed as means ± SEM (error bars). Statistical analyses were performed with one-way ANOVA. N = 6.
