Figure 4 | Scientific Reports

Figure 4

From: The effect of non-thermal atmospheric plasma on the production and activity of recombinant phytase enzyme

Figure 4The alternative text for this image may have been generated using AI.

(A) The result of once treatment with ACP on recombinant phytase production and activity. (a) SDS-PAGE of recombinant phytase produced after once treatment of cell culture with ACP. Lane 1; protein marker, lane 2; control (without ACP treatment), lanes 3–6 belong to treatments with durations of 30, 60, 120, and 240 seconds, respectively. The amount of measured phytase is written on the gel figure. (b) The activity of recombinant phytase produced after once treatment of cell culture with ACP. (B) The result of multiple treatments with ACP on recombinant phytase production and activity. (a) SDS-PAGE of recombinant phytase produced after multiple treatments of cell culture with ACP. Lane 1; protein marker, lane 2; control (without ACP treatment), lanes 3–6 belong to every-day treatments with durations of 30, 60, 120 and 240 seconds, respectively. The amount of measured phytase is written on the gel figure. (b) The activity of recombinant phytase produced after multiple treatments of cell culture with ACP. (**p ≤ 0.01; ***p ≤ 0.001; ****p ≤ 0.0001; paired t-tests; N = 3). Error bars represent standard deviations. Full-length gels are presented in Supplementary Fig. 1.

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