Figure 6
The MR, VP16 and its cellular co-regulator Oct1 synergistically activate HRE promoters. (a) VP16 and the immediate-early (IE) protein ICP4 activate the HRE. VP16 and HSV-1 IE proteins were overexpressed in HeLa cells with the HRE-luc reporter, and luciferase activity was measured and normalised to control transfected cells (pCR3) after 24 hr. Error bars represent the standard error of the mean of three independent experiments carried out in triplicates. Raw data is provided in Table S5. (b) VP16 specifically activates the HRE promoter element. VP16 was overexpressed in HeLa cells with HRE, AP-1, NF-κB, IFN-β or ISRE reporters, and luciferase activity measured and normalised to control transfected cells (pCR3) after 24 hr. Error bars represent the standard error of the mean of three independent experiments carried out in triplicates. Raw data is provided in Table S6. (c) The MR, VP16 and Oct1 synergistically transactivate expression from the HRE. The MR, VP16 and Oct1 were transfected into HeLa cells alone or in combinations with the HRE-luciferase reporter. After 24 hr, luciferase activity was measured and normalised to control transfected cells (pCR3). Error bars represent the standard error of the mean of at least three independent experiments carried out in triplicates. p-values were calculated by unpaired two-tailed t-test for unequal variances. Raw data is provided in Table S7.
