Figure 1 | Scientific Reports

Figure 1

From: A novel somatic mutation of SIN3A detected in breast cancer by whole-exome sequencing enhances cell proliferation through ERα expression

Figure 1

Expression of mRNAs for conventional biomarkers of breast cancers (A-C) Breast cancer tissues (n = 40) were minced and homogenized with a mixer mill, and total RNA was extracted and converted to cDNA by reverse transcriptase. The expressions of ESR1 mRNA (A), PGR mRNA (B) and HER2 mRNA (C) were determined by qPCR using the primers indicated in Supplemental Table 10S. The expression values are shown as comparative values for the mean of the low ESR1 group. The tissues were classified according to the levels of ESR1 mRNA expression into the high ESR1 expression group greater than mean + 2 SD (high ESR1), the low ESR1 expression group less than mean-2SD (low ESR1), or the moderate ESR1 expression group (moderate ESR1) for all samples not characterized in the other groups. The expressions of mRNA were normalized to the expression of GAPDH. The red symbols show the samples used to whole exome sequences (WES). Statistical significance was determined by ANOVA with the Bonferoni post hoc test (*P < 0.05).

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