Figure 5

Intracellular distribution of SIN3A p.Gln944* in breast cancer tissues Tissues with SIN3A-WT (A–C) or SIN3A c.2830 C>T (D–F) were fixed with 4% paraformaldehyde, and cut into 4 µm thick sections after embedding in paraffin blocks. The sections were stained with hematoxylin and eosin (A–D), and then stained with anti-SIN3A antibody for the N-terminal region concurrently with counterstaining with hematoxylin to detect the nuclei (B–F). The boxed regions in the pictures stained with anti-SIN3A antibody (B–E) are magnified 4-fold (C–F). The arrows indicate the typical nuclear regions. The scale bars are 200 µm (A–E) and 50 µm (C–F). For the quantitative analysis of the areas of the nuclear regions and SIN3A localization on the tissues slides, breast cancer tissues containing SIN3A-WT (BC74) or SIN3A c.2830 C>T (BC76) were fixed with 4% paraformaldehyde, embedded in paraffin, and sliced into 4 µm thick sections. The sections were independently stained with hematoxylin to detect nuclei, eosin to detect the cells, or anti-SIN3A antibody. The areas of the nuclear regions in 50 cells (G) and the total intensities of SIN3A in each region in 20 cells (H) were determined using Metamorph software. The data represent the means ± SE. Statistical significances were determined by two-tailed Student’s t test (*P < 0.05).