Figure 4
Comparison on the relative abundance of the five bacterial species in the mock community between the observed and the expected microbiome structures. (A) Relative abundance of the five bacterial species from each sample. (B) Comparison among the extraction methods. (C) Comparison among the targeted hypervariable regions. E. faecalis and S. oralis were over-represented as compared to the expected relative abundance, while L. fermentum and S. mutans were under-represented. A. viscosus was not consistently represented as compared to the expected result. M5 and M6 exhibited the best concordance with the expected distribution, followed by M3. P1 (V1_V3) performed better by producing results closer to the expected relative abundance of A. viscosus than V3-V4 and V4-V5, whereas V3-V4 and V4-V5 generated results that are in closer concordance with the expected abundance of S. mutans. Distinct letters denote significant difference between groups (p < 0.05, Wilcoxon sum-rank test).
