Table 3 The 16S ribosomal RNA hypervariable regions tested in this study.

From: Impact of DNA extraction method and targeted 16S-rRNA hypervariable region on oral microbiota profiling

Region

Forward sequence

Reverse sequence

V1-V3 (P1)

TGGAGAGTTTGATCCTGGCTCAG

TACCGCGGCTGCTGGCAC

V3-V4 (P2)

CCTACGGRRBGCASCAGKVRVGAAT

GGACTACNVGGGTWTCTAATCC

V4-V5 (P3)

GTGYCAGCMGCCGCGGTAA

CTTGTGCGGKCCCCCGYCAATTC