Table 3 List of materials used for the preparation of 1000 mL of a revised simulated body fluid adjusted with albumin (r-SBFA) where the pH was adjusted to be 7.5, using approximately 0.8 mL 1.0 N NaOH. The composition of the r-SBF without albumin was adopted from Oyane et al.63.

From: The nanomolar sensing of nicotinamide adenine dinucleotide in human plasma using a cycling assay in albumin modified simulated body fluids

Reagent

Supplier

Amount (g)

NaCl

SIGMA, USA, Catalog No: DE71376

5.403

NaHCO3

SIGMA, USA, Catalog No: DES5761

0.740

Na2CO3

SIGMA, USA, Catalog No: DES7795

2.046

KCl

ROTH, GER, Catalog No: 6781.1

0.225

KH2PO4

ROTH, GER, Catalog No: 3904.1

0.138

MgCl2⋅6H2O

ROTH, GER, Catalog No: HN03.1

0.311

HEPES

ROTH, GER, Catalog No: 9105.4

11.928

CaCl2⋅2H2O

ROTH, GER, Catalog No: HN04.1

0.388

Na2SO4

SIGMA, USA, Catalog No: S6547

0.072

BSA

SERVA, GER, Catalog No: 47330.03

40

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