Table 5 Dichotomous pH extraction procedure to extract purified versions of either of the pyridine nucleotides, that is, NAD+ or NADH in consideration of Lowry et al.62 and Lilius et al.25 in three fold repetition.

From: The nanomolar sensing of nicotinamide adenine dinucleotide in human plasma using a cycling assay in albumin modified simulated body fluids

Extraction Buffer added (mL)

NAD+ extraction (pH)

NADH extraction (pH)

0 mL (plasma pH)

7.84 ± 0.05

7.89 ± 0.03

1 mL

4.96 ± 0.06

10.29 ± 0.19

2 mL

3.87 ± 0.05

11.48 ± 0.05

3 mL

2.10 ± 0.03

12.22 ± 0.03

4 mL

1.47 ± 0.02

12.58 ± 0.05

5 mL

1.24 ± 0.01

12.67 ± 0.02

6 mL

1.11 ± 0.01

12.81 ± 0.02

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