Figure 3 | Scientific Reports

Figure 3

From: An atypical RNA silencing suppression strategy provides a snapshot of the evolution of sweet potato-infecting potyviruses

Figure 3

Schematic representation of PPV-derived viral constructs. (a) Detailed view of the cloning site of the modified plasmid pPPV∆HC. HCPro linker included to facilitate PPV P1 autocleavage (round arrow) is marked with a dotted box. SwaI site engineered for cloning of the desired genes is marked in grey. NIa cleavage site is indicated with a striped box and a striped vertical arrow. (b) Full-length cDNA clones. Grey horizontal arrows represent the 35S promoter. Round arrow marks the autocatalytic cleavage of PPV P1. NIa cleavage site is marked with a striped box and a striped vertical arrow. The GFP coding sequence inserted between NIb and CP cistrons is marked as a green box.

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