Figure 1 | Scientific Reports

Figure 1

From: Autologous cell lines from circulating colon cancer cells captured from sequential liquid biopsies as model to study therapy-driven tumor changes

Figure 1

Blood samples collection for the establishment of CTC-derived colospheres and timeline of CTC line derivation from sequential blood samples of patient 044. (A) CTC number (assessed with the CellSearch system) in the blood sample of the 168 patients with metastatic colon cancer at V1 (before any treatment); (B) Nine colon CTC lines were established from blood samples of patient 044 at different time points: before treatment initiation (CTC-MCC-41 line), at the time of the second relapse at the end of second-line therapy (CTC-MCC-41.4 line), and at the time of the third relapse before the patient death (CTC-MCC-41.5 A-G lines); D, day; C, treatment cycle; (C) Correlation between CTC number detected with the CellSearch system and in vitro colosphere formation (in blue) for patient 044; CellSearch cell images representative of CTC morphology are shown for the blood samples from which CTC lines could be derived (cells in green). The red line shows the cut-off of approximately 300 cells per 7.5 mL of blood required for in vitro CTC expansion.

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