Figure 5 | Scientific Reports

Figure 5

From: Autologous cell lines from circulating colon cancer cells captured from sequential liquid biopsies as model to study therapy-driven tumor changes

Figure 5

Hierarchical clustering of the nine colon CTC lines relative to the HT-29 and SW620 colorectal cancer cell lines and leukocytes. The expression of 34 mRNAs (Supplementary Table S2) was analyzed in the nine colon CTC lines, HT-29 cells (from a primary colorectal tumor), and SW620 cells (from a lymph node metastasis of colon cancer). These transcripts covered different properties of tumor cells: epithelial (ECAD, CK19, EPCAM), mesenchymal (NCAD, VIM, FN1), stemness (CD133, ALDH1), angiogenesis (VEGF), proto-oncogene (MET), osteomimicry (OPG, BMP7), EMT (SNAIL, TWIST, WNT, DKK1), immune system (IL33, DEFA6, CEACAM1), apoptosis (CCND2, BCL11A, TNFRS1B), DNA repair (GAL, SMARCA1), cell growth (BST2, SEMA6A), cell interactions (ADAMTS6, GJB6, GATA2, TGFB2) and energy metabolism (ABCB1, PTGS2). B2M (housekeeping gene) and CD45 (leukocyte marker) were used as reference genes. Leukocyte cDNA was used as negative control. Results were normalized to the expression level of the reference B2M gene. Cell lines were clustered based on their expression profile.

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