Figure 6 | Scientific Reports

Figure 6

From: Autologous cell lines from circulating colon cancer cells captured from sequential liquid biopsies as model to study therapy-driven tumor changes

Figure 6

Protein expression analysis in the colon CTC lines. (A). Flow cytometry experiments. The expression of a set of proteins was analyzed using different fluorescent antibodies to characterize the phenotype of each CTC line: epithelial (EpCAM, CK19, EGFR), mesenchymal (vimentin, N-cadherin), stemness (CD133, CD44v6, CD44/CD24 ratio), angiogenic (VEGF), immune system escape (PanCD66) and hematopoietic (CD45). The histograms at the bottom show the expression levels (from negative to +++) used to describe the results. (B). Immunofluorescence experiments. Representative images of CTC-MCC-41, CTC-MCC-41.4 and CTC-MCC-41.5G single cells and colospheres showing co-expression of PanCD66 (CEACAM) and CK20, or CD44 and CK19. Nuclei were stained with DAPI (blue). PE, phycoerythrin; FITC, fluorescein isothiocyanate.

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