Figure 3
Biofilm production and PNAG content in biofilm. Biofilm (A) and PNAG in biofilm (B) was measured after 24 h growth in TSB media in a 96-well plate static biofilm assay. Un-inoculated medium served as a cut-off value (ODc) for the quantitative classification of biofilm and PNAG production in no, weak, moderate and strong producers based on their average OD values. Each bar represents the arithmetic mean ± standard deviation (SD) from three independent experiments. (*p < 0.05, **p < 0.01; ***p < 0.001; ****p < 0.0001).
