Figure 5
The effect of intraventricular ketamine application on in vivo calcium activity. (a) Time-lapse images of Fluo-4 fluorescence absolute ΔF/F values before injection. (b) Red circles are regions of interest on identified loaded cells in a. Scale bars correspond to 50 µm. (c) Fluorescence intensity traces in the identified cells in b. (d) Time-lapse images of Fluo-4 fluorescence showing the ΔF/F values after intraventricular injection of 50 mg/ml ketamine. (e) Red circles are regions of interest on identified loaded cells in d. Scale bars correspond to 50 µm. (f) Fluorescence intensity traces in the identified cells in e. The slight variations in the signal are rising from the motion artefacts, as could be appreciated from synchronicity of the disturbances.
