Figure 4 | Scientific Reports

Figure 4

From: A novel alkaline protease from alkaliphilic Idiomarina sp. C9-1 with potential application for eco-friendly enzymatic dehairing in the leather industry

Figure 4

SDS-PAGE analysis of recombinant AprA and AprA-PPC. (A) The supernatant of crude extract from E. coli BL21 (DE3) PlysS expression strains. Lane 1, control containing pET28a without 10 mM PMSF; lane 2, AprA crude extract without 10 mM PMSF; lane 3, AprA-PPC crude extract without 10 mM PMSF; lane 4, control containing pET28a with PMSF; lane 5, AprA crude extract with PMSF; lane 6, AprA-PPC crude extract withPMSF. (B) The purified enzymes. Lane 7, purified recombinant AprA with 10 mM PMSF; lane 8, purified recombinant AprA-PPC with 10 mM PMSF; lane 9, purified recombinant AprA without PMSF; lane 10, purified recombinant AprA-PPC without PMSF. Lane M, molecular weight marker.

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