Figure 6

Effects of temperature on AprA and AprA-PPC activity and stability with or without Ca2+. (A) The optimal temperature for AprA activity; (B) The optimal temperature for AprA-PPC activity; (C) Thermal stability of AprA; (D) Thermal stability of AprA-PPC. Filled symbols indicated assay with 5 mM Ca2+, whereas the empty symbols indicated assay without Ca2+. The thermal stability was determined by assaying the residual activity of enzyme under standard condition after incubation at different temperature for various time intervals. The measurements were performed in three independent experiments. Error bars represent standard deviations.