Table 2 Effects of metal ions on activity of recombinant AprA and AprA-PPC.
Metal ions (5 mM) | Relative Activity (%) | Metal ions (5 mM) | Relative Activity (%) | ||
|---|---|---|---|---|---|
AprA | AprA-PPC | AprA | AprA-PPC | ||
Control | 100.0 | 100.0 | Fe3+ | 74.4 ± 1.7 | 67.0 ± 1.1 |
K+ | 101.4 ± 1.5 | 102.4 ± 3.1 | Ba2+ | 107.8 ± 4.1 | 110.0 ± 2.9 |
Na+ | 100.2 ± 1.1 | 98.9 ± 2.5 | Mn2+ | 119.7 ± 3.2 | 116.4 ± 3.1 |
Al3+ | 102.6 ± 1.6 | 99.7 ± 2.1 | Co2+ | 112.0 ± 4.0 | 107.7 ± 2.7 |
Ca2+ | 115.4 ± 2.1 | 104.7 ± 1.8 | Zn2+ | 66.2 ± 2.2 | 62.9 ± 1.9 |
Li+ | 100.9 ± 3.2 | 102.9 ± 3.1 | Ag+ | 68.8 ± 3.1 | 71.7 ± 2.0 |
Cu2+ | 122.8 ± 3.8 | 107.8 ± 2.3 | Fe2+ | 66.3 ± 1.4 | 63.2 ± 1.8 |
Mg2+ | 120.1 ± 2.4 | 115.2 ± 1.9 | Ni2+ | 35.1 ± 1.0 | 33.6 ± 1.6 |
Hg2+ | 10.1 ± 0.6 | 9.5 ± 0.4 | Pb2+ | 12.1 ± 0.7 | 11.7 ± 0.8 |
