Table 3 Effect of detergents, oxidizing and reducing reagents and protease inhibitors on enzyme activity.
Detergent | Concentration | Relative activity of AprA (%) | Relative activity of AprA-PPC (%) |
|---|---|---|---|
Control | — | 100.0 | 100.0 |
Tween-20 | 1% (v/v) | 92.6 ± 3.1 | 78.2 ± 1.0 |
Tween-40 | 1% (v/v) | 92.5 ± 2.4 | 90.9 ± 1.4 |
Tween-80 | 1% (v/v) | 82.5 ± 2.1 | 75.2 ± 0.9 |
Triton X-100 | 1% (v/v) | 91.4 ± 3.0 | 83.9 ± 1.2 |
Triton X-155 | 1% (v/v) | 99.1 ± 2.9 | 86.9 ± 1.7 |
H2O2 | 1% (v/v) | 129.3 ± 4.2 | 121.5 ± 2.3 |
2% (v/v) | 126.1 ± 2.8 | 117.5 ± 2.1 | |
5% (v/v) | 97.3 ± 1.9 | 84.7 ± 0.8 | |
SDS | 1% (w/v) | 75.2 ± 2.6 | 71.9 ± 1.6 |
JFC-2 | 1% (v/v) | 90.6 ± 1.2 | 103.8 ± 2.2 |
Peregal-O | 1% (w/v) | 88.6 ± 1.1 | 98.5 ± 2.0 |
β-Mercaptoethanol | 1% (v/v) | 87.6 ± 1.3 | 99.1 ± 1.4 |
PMSF | 5 mM | 0.0 | 0.0 |
EDTA | 2 mM | 88.9 ± 0.9 | 88.8 ± 1.2 |
5 mM | 42.4 ± 1.0 | 46.5 ± 0.4 | |
10 mM | 13.6 ± 0.2 | 48.5 ± 0.7 | |
EGTA | 2 mM | 85.4 ± 0.9 | 85.4 ± 1.1 |
5 mM | 37.5 ± 0.2 | 45.3 ± 0.8 | |
10 mM | 7.4 ± 0.1 | 14.7 ± 0.5 |
