Table 6 Comparison of secretory expression of alkaline proteases in different strains.
Gene source and references | Expression strains | Expression vector | Culture time (h) | Activity (U ml−1) | Productivity (U ml−1 h−1) |
|---|---|---|---|---|---|
Idiomarina sp. | B. subtilis WB600 | pMA5 | 48 | 4935.5 | 102.8 |
B. pumilus 56 | B. subtilis WB600 | pSU | 48 | 32.8 | 0.7 |
B. subtilis 65 | B. subtilis DB100 | pUB110 | 10 | 26.0 | 2.6 |
B. pumilus 80 | B. subtilis DB430 | pBSMuL2 | 72 | 5800.0 | 80.6 |
Bacillus sp.79 | B. subtilis DB104 | pAH101NC | 24 | 30.0 | 1.3 |
B. subtilis 57 | B. subtilis Bios11 | pUB110 | 11 | 24.5 | 2.2 |
A. oryzae 81 | P. pastoris GS115 | pPIC9K | 120 | 47.5 | 0.4 |
B. stearothermophilus 12 | P. pastoris GS115 | pGAPZαB | 72 | 41.3 | 0.6 |
A. nidulans 58 | P. pastoris X33 | pPICZαA | 48 | 1.0 | <0.1 |
B. cereus 82 | P. pastoris X33 | pPICZαA | 72 | 122.6 | 1.7 |
A. sojae 83 | P. pastoris KM71 | pPIC9K | 72 | 400.4 | 5.6 |
B. stearothermophilus 27 | E. coli XL1-Blue | pTrcHis | 24 | 22 | 0.9 |
S. maltophilia 43 | E. coli BL21-Gold | pMS470Δ8 | 20 | 60.0 | 3.0 |
Metagenome45 | E. coli BL21-Gold | pMS470Δ8 | 28 | 27.0 | 1.0 |
A. pullulans 23 | Y. lipolytica Po1h | pINA1317 | 96 | 49.5 | 0.5 |
A. oryzae 84 | S. cerevisiae NA87-11A | pMA56 | 18 | 1.7 | 0.1 |
Fusarium sp.85 | S. cerevisiae AH22R | pGAN1 | 72 | 15.1 | 0.2 |
A. oryzae 86 | Z. rouxii ATCC13356 | pZAP101A | 168 | 166.1 | 1.0 |
