Figure 7
From: Acid bone lysate activates TGFβ signalling in human oral fibroblasts
Acid bone lysate (ABL) obtained from mandible, calvaria and tibia produces an equivalent gene expression and TGF-β activity is highly dependent on hydrochloric acid concentration. ABL obtained from different sources (mandible, calvaria and tibia) produces a similar response on selected genes (a). ABL prepared with 0.1 N hydrochloric acid induces a higher TGF-β activity at mRNA level (b). Immunoassay of IL11 confirmed the higher activity of TGF-β at protein level with 0.1 N ABL (c). N = 3–5. Data represent the mean ± SD. P > 0.05, by Kruskal-Wallis test with Dunn’s multiple comparisons correction, *P < 0.05, **P < 0.01, by two-tailed Mann-Whitney test.
