Figure 1 | Scientific Reports

Figure 1

From: Overexpression of CD98 in intestinal epithelium dysregulates miRNAs and their targeted proteins along the ileal villus-crypt axis

Figure 1

Isolation of ileal epithelial cells from villus and crypt fractions. Ileal epithelial cells were isolated from villi and crypt of FVB (WT) and FVB Villin-hCD98 (Tg) mice at 6–9 weeks of age using a previously described method with modification (a). Removed tissue were washed in series of buffer and collected to yield fractions with gradient presence of villi, villi-crypt, or crypt. Fractions were visualized by light microscopy at 10x magnification (b, scale bar: 100 µm).

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