Figure 2 | Scientific Reports

Figure 2

From: Overexpression of CD98 in intestinal epithelium dysregulates miRNAs and their targeted proteins along the ileal villus-crypt axis

Figure 2

Proteins associated with villus and crypt are disrupted in CD98 overexpressing mice. Total RNA were extracted and pooled from ileal villi and crypt fractions from IEC-specific hCD98 overexpressing transgenic (Tg) and FVB wildtype (WT) mice. Expression levels of villus marker, mPepT1, were examined from different villus and crypt fractions (b, n = 6; WT villus vs WT crypt: t = 3.095, P < 0.0102; Tg villus vs Tg crypt: t = 8.346, P < 0.001) with representative expression levels shown across all fractions (a). Expression level of crypt marker, Lgr5, was also assessed (e, n = 6; WT villus vs WT crypt: t = 3.046, P < 0.0111; Tg villus vs Tg crypt: t = 3.541, P < 0.0046) with representative expression levels shown across all fractions (d). Other markers associated with villus and crypt were also examined across all fractions collected (c, Cdx2; f, mCD98; g, hCD98; h, Muc2; i, Villin).

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