Figure 1
The three phospho-serines surrounding the HMG-box are important for SOX11’s transcriptional activity. (a) Representation of the phosphorylated serine residues on SOX11 WT and Table presenting the amino acids that are exchanged on each mutant. S = Serine (wild type), A = Alanine (NON-phosphorylatable aa) and D = Aspartate (aa that mimics phosphorylation). (b) Luciferase assays using a SOX11-responsive minimal promoter n = 3, performed in HEK293T cells (Student’s unpaired T-test: WT vs NON p = 0.021, WT vs MIMIC p = 0.027). (c) Luciferase assays using a SOX11-responsive minimal promoter n = 3, performed in HEK293T cells (Student’s unpaired T-test: WT vs N3W7 p = 0.021, WT vs M3W7 p = 0.022, WT vs W3N7 p = 0.17, WT vs W3M7 p = 0.81). The data are presented as mean ± SEM.
