Table 1 Primary antibodies.

From: Phosphorylation of the neurogenic transcription factor SOX11 on serine 133 modulates neuronal morphogenesis

Antigen

Species

Company

Working Dilution

Catalog number

RRID

SOX11

Rabbit

Abcam

1:500

ab134107

AB_2721126

Doublecortin (DCX)

Goat

Santa Cruz

1:500

SC8066

AB_2088494

Mouse SOX11*

Rat

In house

1:100

—

AB_2732801

GFP

Chicken

Aves Labs

1:500

GFP-1020

AB_10000240

pCREB(S133)

Rabbit

Cell Signaling

1:500

9198 S

AB_2561044

PKA Catalytic Subunit

Rabbit

Abcam

1:500

ab75991

AB_1524202

PKA Catalytic Subunit

Mouse

Santa Cruz

1:500

sc28315

AB_628136

RFP

Rat

Chromotek

1:500

5F8-100

AB_2336064

  1. *The monoclonal Sox11 antibody (clone 19B2, IgG2a, AB_2732801) was generated in house (E. Kremmer, Service Unit Monoclonal Antibodies, Helmholtz-Zentrum München) as previously described51. Briefly, as antigen, HIS-GST fusion protein using the pETM30 vector (provided by the EMBL) was generated encompassing the homologous mouse sequence of a human SOX11 PrEST (https://www.proteinatlas.org)52 and corresponding to the amino acids 189-301 of mouse SOX11. The protein was recombinantly expressed in E. coli. The soluble protein fraction was purified via the N-terminal 6xHis tag using Ni-NTA Agarose (Qiagen) according to standard protocols provided by the manufacturer and followed by dialysis against PBS to remove the imidazole. Rats (Lou/C) were immunized with purified His-GST Sox11-189-301. Fusion was performed with the myeloma cell line P3X63Ag8.653. After generation of hybridomas, selected clones were subcloned till stability.
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