Figure 3
Sensory neurons from PVCre;STP-YFP;SOD1G93A mice show decreased axonal growth rate. Explant cultures of PVCre;YFP and PVCre;YFP;SOD1G93A DRGs were live-imaged at 72, 96 and 144 hours post-plating, and rates were determined based on the initial length at 72 hours (A). In control sensory neurons, the axonal growth rate remains consistent between 96 hours (B) and 144 hours (B’), while PVCre;STP-YFP;SOD1G93A cultures show a significant decrease in growth rate between 96 hours (C) and 144 hours (C’,D). Arrows are marked in the same position at 96 hours (B,C) and 144 hours (B’, C’). hSOD1 mRNA is found at similar levels in DRGs from PVCre;YFP;SOD1G93A compared to DRGs from SOD1G93A mice and as expected not detected (ND) in wild type mice (E). Control n = 3; 130 days old, PVCre;STP-YFP;SOD1G93A n = 3; 130 days old. At least four individual neurites were traced per explant with at least two explants examined per mouse. Represented values are the average of all animals per genotype. Only male mice were used for this experiment. Expression is normalized to GAPDH and relative to PVCre;YFP;SOD1G93A. Scale Bar = 100 µm. Error bar = SEM. P-value = **<0.01, ***<0.001.
