Figure 4
Sensory neurons from SOD1G93A and TDP43A315T mice have decreased neurite length and complexity following vincristine exposure. Dissociated cultures of DRGs from control (A), SOD1G93A (B), and TDP43A315T (C) mice were treated with 0.01 µM or 0.5 μM vincristine for 4 hours beginning 24 hours post-plating. Following exposure to 0.5 μM vincristine for 4 hours, control, SOD1G93A and TDP43A315T sensory neurons have significantly decreased neurite length compared to vehicle treated neurons (D–F). Sensory neurons from SOD1G93A mice treated with 0.01 µM vincristine have significantly decreased neurite intersections between 110 and 130 µm from the soma compared to control (G). Sensory neurons from TDP43A315T mice treated with 0.01 µM vincristine have significantly decreased neurite intersections between 30 and 330 µm from the soma compared to control (G). Exposure to 0.5 μM vincristine for 4 hours significantly decreases the number of neurite intersections between 50 and 290 µm in SOD1G93A sensory neurons compared to control (H). Sensory neurons from TDP43A315T mice treated with 0.01 µM vincristine have significantly decreased neurite intersections between 30 and 310 µm from the soma compared to control (H). Arrows (B,C) indicate blebbing neurites. Control n = 3; 130 days old, SOD1G93A n = 3; 130 days old, and TDP43A315T n = 4; 130 days old. Only male mice were used for this experiment. At least 5 sensory neurons were analyzed per animal. Represented values are an average of values from all animals per condition. Scale Bar = 100 µm. Error bar = SEM. (D–F) P-value = *<0.05, (G–H) P-value = *<0.05 compared to control, +<0.05 compared to SOD1G93A.
