Figure 4 | Scientific Reports

Figure 4

From: Fast Optical Sectioning for Widefield Fluorescence Mesoscopy with the Mesolens based on HiLo Microscopy

Figure 4

Contrast improvement of Mesolens HiLo over widefield. (a) is a widefield image, (b) the HiLo processed image and (c) are the respective plots of vertical line (1). The images are of the same region of the spine as in Fig. 2 but at a different z-position. Z-position was chosen to specifically highlight the contrast improvement. Contrast was calculated by dividing peak intensity value by the minimum value (out-of-focus background). Results were 2 for the widefield image and 12 for the HiLo processed image, i.e. a six-fold increase in contrast. Scale bar applies to both images.

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