Figure 5
Comparison between HiLo processed optical section (a) and confocal laser scanning microscopy optical section (b). The two sections are approximately in the same focal plane of the same zebrafish which was used for Figs 2–4. However, the confocal data was obtained in epi-illumination while the HiLo data was taken with transmission illumination. The difference in illumination accounts for the major discrepancies in the observed structure. Furthermore, the confocal data had more even intensity across the field of view.
