Figure 6 | Scientific Reports

Figure 6

From: Receptor-Ligand Interaction Mediates Targeting of Endothelial Colony Forming Cell-derived Exosomes to the Kidney after Ischemic Injury

Figure 6

Internalization of endothelial colony forming cell (ECFC)-derived exosomes by hypoxic HUVECs is blocked by plerixafor and neutralizng antibody to SDF-1α. (a) Immunoblot shows expression of CXCR4 in ECFCs and their derived exosomes (EX). (b) Graph and representative images of HUVECs incubated for 6 hrs with 20 μg/ml of PKH26-labeled exosomes (red) in normoxia (C), hypoxia (C*), hypoxia with 100 μM plerixafor (Plerixafor), hypoxia with 10 μg/ml of neutralizing antibody to SDF-1α (SDF-1α) and Isotype control (IgG). HUVEC nuclear counterstaining was performed using Hoescht stain (blue). +P < 0.001 vs C, *P < 0.001 vs C*, by one-way ANOVA, n = 4.

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