Figure 1
From: Dynamic recognition and linkage specificity in K63 di-ubiquitin and TAB2 NZF domain complex
Crystal structures of the di-Ub/NZF complexes and sequence alignment of the NZF domains. (A) K63 di-Ub/TAB2 NZF (PDB: 2wwz). (B) Linear di-Ub/HOIL-1L NZF Δtail (PDB: 3b0a). Here, Δtail indicates that the NZF domain does not contain the C-terminal helix of HOIL-1L (224–249). (C) K33 di-Ub/TRABID NZF1 (PDB: 5af6). (D) Mono Ub/NPL4 NZF (PDB: 1q5w). In (B–D), TAB2 NZF (cyan) is drawn after superimposition to the core region (1–70) of the distal Ub (left moiety of di-Ub) or mono-Ub. (E) The sequences of the NZF domains used in this study were aligned. ZRAN1 corresponds to TRABID. Red characters indicate the amino acids which are conserved in all four proteins.
