Figure 7
From: Flow cytometric analysis of Xenopus laevis and X. tropicalis blood cells using acridine orange
Analysis of peripheral blood derived from anaemic X. laevis. (A) Erythrocyte counts (left panel) and haemoglobin (Hb) concentration (right panel) in peripheral blood after the administration of phenylhydrazine (PHZ; 25 mg/kg body weight, intraperitoneally, day 0). All values are shown as the mean ± SD. **p < 0.01 vs control (n = 4). (B) The LP3 fraction was analysed to determine the F530 and F695 fluorescence intensity. The F695high population of the LP3 fraction was markedly increased after PHZ administration. (C) Comparison of immature erythrocyte counts between the manual method (left panel) and flow cytometry (right panel). *p < 0.05 vs. control (n = 4).
