Figure 1
From: Target site specificity and in vivo complexity of the mammalian arginylome
Four mouse ATE1 isoforms have different, partially overlapping specificity toward their N-terminal target sites. Cropped image of the autoradiogram showing Arg incorporation as the 14C signal (gray or black after the film exposure). ATE1 isoforms are indicated on the left. In each ATE1 isoform group, two rows show the two repeats of the same experiment, performed using the arrays synthesized in duplicates on the same membrane and analyzed in duplicates in the arginylation assays (see Fig. S1 for the original uncropped image). The peptide sequences are shown underneath, with the target residue underlined. The peptide backbone was selected based on the N-terminal beta actin peptide, as explained in the text.
