Figure 4
From: Target site specificity and in vivo complexity of the mammalian arginylome
Arginylation of “non-canonical” N-terminal residues. (A) Arginylation assay with synthesized peptides in solution with varied residues in the N-terminal position (sequences are indicated on the x axis). Y axis shows [3H-R] incorporation signal, normalized to that of the D-containing peptide. Error bars represent SEM, n = 3 independent repeats. See Dataset 1 for the mass spectra of the D- and C-peptides. (B) Arginylation assay with the peptide containing N-terminal D (as a positive control) as well as unoxidized and oxidized C (sequences are indicated on the x axis; oxidation is denoted as (O3). Error bars represent SEM, n = 3 independent repeats.
